Erythrosin B Assay

Erythrosin B is a biosafe and non-toxic colorimetric dye that can be used as an alternative to Trypan Blue for cell counting and viability assessment. Evaluation of cell viability using Erythrosin B dye exclusion is based on a similar principle to Trypan Blue, that intact cell membranes exclude the polar molecules whereas compromised membranes do not. Live cells remain unstained while dead cells are stained and have a darker appearance.